In Nikhat Parveen alias Khusboo Khatoon v. Rafique alias Shillu, decided on April 22, 2026 (cited as 2026 LSI (SC) 100), the Supreme Court of India held that conclusive DNA evidence proving non-paternity prevails over the statutory presumption of legitimacy under Section 112 of the Indian Evidence Act, 1872, and consequently no maintenance obligation can arise where biological paternity stands disproved. The bench emphasised that scientific certainty must supersede legal fiction wherever incontrovertible biological proof contradicts a statutory presumption. Section 112, which presumes a child born during the continuance of a valid marriage to be the legitimate child of the husband unless non-access during conception is proved, has long been treated as nearly conclusive. The judgment crystallises a two-decade judicial evolution from protecting social legitimacy at all costs towards prioritising evidentiary accuracy in determining parental responsibility, while preserving procedural safeguards: DNA testing must follow strict consent and chain-of-custody protocols, and unjustified refusal to undergo lawful testing may invite an adverse inference. The ruling affects pending Section 125 CrPC and Section 144 BNSS maintenance proceedings nationwide, including those before family courts, and intersects with constitutional guarantees of dignity under Article 21 and the right against self-incrimination under Article 20(3).